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Hoechst 33342 trihydrochloride (CAS 23491-52-3)Synonym:BisbenzimideApplication:An A:T-specific DNA minor groove ligand used for visualizing cellular DNACAS Number:23491-52-3Purity:95%Molecular Weight:561.9Molecular Formula:C27H28N6O 3HClRefer to Certificate of Analysis for lot specific data (including water content).
Description
Hoechst 33342 trihydrochloride is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin ( sc-200923) has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342’s fluorescent capability, the chemical has also had varying effects on differing cell types. The stain has been shown to induce cell differentiation, bind reversibly to Friend erythroleukemia cells, inhibit Topo I (topoisomerase I), induce apoptosis in HL-60 cells, and in junctional sarcoplasmic reticulum cells regulate Ca 2+ efflux and affect cell differentiation.
Technical Information
Physical State: |
Solid |
Solubility: |
Soluble in water (20 mg/ml). Precipitates in phosphate buffered solutions |
Storage: |
Store at -20° C |
Fluoresence: |
λex 346 nm, λem 460 nm |
Safety and Reference Information
RTECS: |
DT4200000 |
PubChem CID: |
16760503 |
Merck Index: |
14: 10367 |
MDL Number: |
MFCD00012678 |
SMILES: |
CCOC1=CC=C(C=C1)C2=NCC3=C(N2)C=CC(=C3)C4=NCC5=C(N4)C=CC(=C5)N6CCN(CC6)C.Cl.C l.Cl |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
1. Latt, S.A., et al. 1976. J. Histochem. Cytochem. 24: 24-33. PMID: 943439 2. Arndt-Jovin, D.J., et al. 1977. J. Histochem. Cytochem. 25: 585-589. PMID: 70450 3. Lydon, M.J., et al. 1980. J. Cell. Physiol. 102: 175-181. PMID: 6154714 4. Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. U.S.A. 78: 363-367. PMID: 6941251 5. Gregoire, M., et al. 1984. Exp. Cell Res. 152: 38-46. PMID: 6201374 6. Crissman, H.A., et al. 1987. Exp. Cell Res. 173: 256-261. PMID: 2960553 7. Loontiens, F.G., et al. 1990. Biochemistry. 29: 9029-9039. PMID: 1702995 8. Steuer, B., et al. 1990. Exp. Cell Res. 186: 149-157. PMID: 2298232 9. Crissman, H.A., et al. 1990. Methods Cell Biol. 33: 97-103. PMID: 1707496 10. Ormerod, M.G., et al. 1993. Cytometry. 14: 595-602. PMID: 8404365 11. Wong, S.S., et al. 1994. Biochem. Pharmacol. 47: 827-837. PMID: 7510966 12. Belloc, F., et al. 1994. Cytometry. 17: 59-65. PMID: 7528124 13. Zhang, X. and Kiechle, F. 2001. Ann. Clin. Lab. Sci. 31: 187-198. PMID: 11337909
Информация для заказа:Область использования: | Производство: | Santa Cruz | Метод: | | Объем: | 100 мг | Кат. номер: | SC-200 908 | Цена (с НДС 20%): | | В корзину | Наименование: Hoechst 33342 trihydrochloride (CAS 23491-52-3). Примечание: |
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