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Propidium Iodide
| Synonym: |
2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide |
| Application: |
A membrane-impermeable fluorescent nucleic acid stain |
| CAS Number: |
25535-16-4 |
| Purity: |
≥95% |
| Molecular Weight: |
668.39 |
| Molecular Formula: |
C27H34I2N4 |
| Refer to Certificate of Analysis for lot specific data (including water content). |
Description
Propidium iodide is a laboratory reagent for the fluorescent staining of nucleic acids. Useful for flow cytometry for staining apoptotic cells and nuclei. Because it is membrane impermeable the state of the cell determines the ability of propidium iodide from staining viable and apoptotic cells. Propidium iodide has been used in in combination with Hoechst fluorescent stain ( sc-202503) to observe cell viability via fluorescent microscopy. Propidium iodide is excitable at 536 nm and Emits at 617 nm (red). Technical Information
| Appearance: |
Powder |
| Physical State: |
Solid |
| Solubility: |
Soluble in water (1 mg/ml), DMSO, and methanol (3 mg/ml). |
| Storage: |
Store at 4° C |
| Melting Point: |
220-225 °C |
| Fluoresence: |
λex 536 nm, λem 617 nm |
| IC50: |
Glutamate NMDA receptor: IC50 = 6.8 µM (Rattus norvegicus); Butyrylcholinesterase: IC50 = 12.6 µM (Equus caballus); Acetylcholinesterase: IC50 = 34.6 µM (Bos taurus); Acetylcholinesterase: IC50 = 7.14 µM (human) |
| Ki Data: |
Acetylcholinesterase: Ki= 8.6 µM (Electrophorus electricus); Acetylcholinesterase: Ki= 3.73 µM (Torpedo californica); Acetylcholinesterase: Ki= 2.23 µM (Mus musculus); Acetylcholinesterase: Ki= 7.14 µM (human) |
| pK Values: |
pKa: 1.63 (Predicted) |
Safety and Reference Information
| WGK Germany: |
3 |
| RTECS: |
SF7949600 |
| PubChem CID: |
104981 |
| MDL Number: |
MFCD00011921 |
| EC Number: |
247-081-0 |
| Beilstein Registry: |
3843838 |
| SMILES: |
CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
1. Tas, J. and Westerneng, G. 1981. J. Histochem. Cytochem. 29: 929-936. PMID: 6168679
2. Darzynkiewicz, Z., et al. 1992. Cytometry. 13: 795-808. PMID: 1333943
3. Darzynkiewicz, Z., et al. 1998. Adv. Biochem. Eng. Biotechnol. 62: 33-73. PMID: 9755640
4. Darzynkiewicz, Z. and Bedner, E. 2000. Meth. Enzymol. 322: 18-39. PMID: 10914002 Информация для заказа:| Область использования: | Производство: | Santa Cruz | | Метод: | | | Объем: | 50 мг | | Кат. номер: | sc-3541 | | Цена (с НДС 20%): | | В корзину  |  Наименование: Propidium Iodide.
Примечание: Видоспецифичность: .
Описание: Десикант, слабо чувствительный.
Приложение: .
Доставка на голубом льду, хранение при 4°C.
Подробное описание (на английском языке). |
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