Alkaline Phosphatase (H-300) / Alkaline Phosphatase Antibody (H-300) rabbit polyclonal IgG

Alkaline Phosphatase (H-300): sc-30203. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Alkaline Phosphatase Antibody (H-300) is a rabbit polyclonal IgG provided at 200 µg/ml

epitope corresponding to amino acids 18-317 mapping near the N-terminus of TNAP of human origin

recommended for detection of TNAP of mouse, rat and human origin and, to a lesser extent, PLAP, ALPPL2 and IAP of human origin and Akp-3, Akp-5 and Akp-6 of mouse and rat origin by WB, IP, IF and ELISA; partially cross-reactive with other alkaline phosphatases ; also reactive with additional species, including equine, canine and bovine

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Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. They are most effective in an alkaline environment and specifically function to remove phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins and alkaloids. There are at least four distinct but related alkaline phosphatases: intestinal (IAP), placental (PLAP), placental-like (ALP-1 or GCAP) and tissue non-specific (TNAP). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Alkaline phosphatases have become useful tools in molecular biology laboratories, removing the phosphate groups on the 5’ end of DNA. Removal of these phosphates prevents the DNA from ligating, thereby preventing DNA degradation. In addition, alkaline phosphatases can also be used as a label for enzyme immunoassays.

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